| 학회 |
한국화학공학회 |
| 학술대회 |
2019년 봄 (04/24 ~ 04/26, 제주국제컨벤션센터) |
| 권호 |
25권 1호, p.395 |
| 발표분야 |
생물화공(Biochemical Engineering) |
| 제목 |
Deletion of a minor global regulator yieP greatly improves the tolerance of Escherichia coli to 3-hydroxypropionic acid |
| 초록 |
3-Hydroxypropionic acid (3-HP) is an important platform chemical which can be converted to many useful chemicals. The toxic effects of 3-HP on cell growth and viability is a serious challenge for its industrial biological production. In this study, a highly 3-HP tolerant strain was developed from Escherichia coli W by adaptive laboratory evolution (ALE). Genome analysis indicated that 13 genes including glpK and yieP were mutated during ALE. For the mutated genes, contribution to 3-HP tolerance was studied by multiplex automated genome engineering (MAGE). Interestingly, a nonsense mutation in yieP alone was fully responsible for the improved tolerance to 3-HP. Mutation or complete deletion of yieP conferred a similar 3-HP tolerance to other E. coli strains. The study on 3-HP tolerance mechanism conferred by ΔyieP is in progress |
| 저자 |
Phu Nguyen-Vo Thuan1, Yunxiao Liang2, Mugesh Sankaranarayanan1, Eunhee Seol2, Ah Yeong Chun2, Somasundar Ashok2, Ashish Chauhan2, Jung Rae Kim2, Seyoung Ko3, Donghyuk Kim3, 박성훈1
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| 소속 |
1Pusan National Univ. / Ulsan National Institute of Science and Technology, 2Pusan National Univ., 3Ulsan National Institute of Science and Technology |
| 키워드 |
생물화학공학 |
| E-Mail |
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| 원문파일 |
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