| 초록 |
Biological production of 3-hydroxypropionic acid (3-HP) from glycerol is possible through two sequential enzymatic reactions, which are catalyzed by a coenzyme B12-dependent glycerol dehydratase and an NAD(P)+-dependent aldehyde dehydrogenase (ALDH), respectively. Pseudomonas denitrificans is considered an ideal host for the production of 3-HP from glycerol, because it can synthesize coenzyme B12 under aerobic conditions where NAD(P)+ is regenerated efficiently. When the enzymes glycerol dehydratase (DhaB) and glycerol dehydratase reactivase (GdrAB2) of Klebsiella pneumoniae were heterologously expressed, P. denitrificans could produce 3-HP from glycerol at 37.7 mM in flask culture. Glucose was needed as carbon and energy sources for cell growth. Overexpression of a heterologous ALDH was not required since unknown ALDH(s) present in P. denitrificans, although its activity was low. The rate of 3-HP production was improved by by overexpressing an ALDH gene (puuC) from K. pneumonia. P. denitrificans oxidized 3-HP to malonate and it was suggested that this activity should be removed for further improvement of the recombinant strain. |
