| 학회 |
한국공업화학회 |
| 학술대회 |
2015년 가을 (11/04 ~ 11/06, 제주국제컨벤션센터(ICCJEJU)) |
| 권호 |
19권 2호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
In vitro and in vivo expression of phospholipase A1 in Escherichia coli |
| 초록 |
Phospholipase A1 (EC 3.1.1.32) is an enzyme that hydrolyzes phospholipid into a fatty acid and a lysophosphoslipid. Phospholipase has potential industrial applications in such fields as bioenergy, food, and pharmaceutical industry. The lipolytic activity of phospholipase, however, causes inhibition of cell growth and hinders its production in the conventional cell-based expression methods. Because it does not require membrane integrity for protein synthesis, cell-free protein synthesis system using cell lysates can offer an alternative route. In this study, we attempted to express the phospholipase A1 gene cloned from the chromosomal DNA of Serratia sp. MK1 either in E.coli cells or a cell-free synthesis system derived from the lysate of the E.coli cells. The phospholipase can be efficiently produced in the cell-free protein synthesis system with higher volumetric productivity compared to the recombinant E.coli cells (370.2 ug/mL vs 115.8 ug/mL, respectively). We expect that the cell-free approach can provide a solution for production of many other enzymes that interfere with the growth and viability of host cells. |
| 저자 |
김동명1, 송재광2, 장연재1, 박유진1, 임혜진2
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| 소속 |
1충남대, 2한국화학(연) |
| 키워드 |
Phospholipase A1; Cell-free protein synthesis; Protein expression
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| E-Mail |
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