| 학회 |
한국재료학회 |
| 학술대회 |
2016년 가을 (11/16 ~ 11/18, 경주 현대호텔) |
| 권호 |
22권 2호 |
| 발표분야 |
B. 나노화학/바이오 재료 분과 |
| 제목 |
Isolation of renal cells from adult rat kidneys and the effect of layer-by-layer assembly nanocoating on the proliferation and differentiation of renal progenitor cells |
| 초록 |
Layer-by-layer self-assembly is a thin film fabrication technique that involves the deposition of alternating layers of oppositely charged biocompatible materials (polycation and polyanion). This technique offers physiological versatility by nanocoating of a single-cell to create a defined microenvironment for cell proliferation and differentiation. In this study we report isolation of progenitor cells from adult rat kidneys and its encapsulation using a layer-by-layer self-assembly technique with polyelectrolytes chitosan/ gelatin and alginate. Analysis of the nanoencapsulated renal progenitor cells showed that the layer-by-layer assembly did not affect the viability, and proliferation and differentiation of the cells for 21 days under 2D culture. Results showed that the single-cell encapsulation of renal progenitor cells was able to protect the cells without interfering with its biological activity and has potential for use in cell delivery where physiological versatility is necessary including, but not limited to the recellularization of acellular tissue constructs. |
| 저자 |
Celine Abueva1, Nathaniel Carpena2, Chan-Mi Park3, Byong-Taek Lee1
|
| 소속 |
1Department of Regenerative Medicine, 2College of Medicine, 3Soonchunhyang Univ. |
| 키워드 |
stem cell; renal cell; single-cell encapsulation; kidney regeneration
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| E-Mail |
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