| 학회 |
한국공업화학회 |
| 학술대회 |
2015년 봄 (04/29 ~ 05/01, BEXCO (부산)) |
| 권호 |
19권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Co-production of hydrogen and ethanol from glucose by the modification of glycolytic pathway |
| 초록 |
In our previous study, co-production of H2 and ethanol was studied by deleting pfkA and pta-ackA. Deletion of these genes accumulated substantial amount of pyruvate as by-products due to the shortage of NADH. To supply more NAD(P)H for ethanol production by activating PP pathway, we overexpressed G6PDH and 6PGDH which are known as limiting enzymes in PP pathway. Over-expression of these enzymes increased co-production yield by reducing pyruvate secretion significantly. This result confirms that carbon flux was diverted to PP pathway thus increasing NAD(P)H availability for co-production. RT-PCR and MFA supported this observed results. Final co-production yield of 1.21 H2/ mol glucose and 1.25 mol ethanol/mol glucose was achieved. |
| 저자 |
SUNDARA SEKAR BALAJI, 설은희, Satish Kumar Ainala, 박성훈
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| 소속 |
부산대 |
| 키워드 |
Hydrogen production; Co-production; ethanol production
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| E-Mail |
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