| 초록 |
To overcome the low yield of H2 production by dark fermentation, co-production of H2 and ethanol by Escherichia coli from glucose, was suggested. In order to increase the cofactor availability for ethanol production, pentose phosphate (PP) pathway was suggested as the major glycolytic route and its activation was studied by constructing various genetic backgrounds in the base strain (E. coli BW25113 ΔhycA ΔhyaAB ΔhybBC ΔldhA ΔfrdAB). Resulting strains could increase the co-production yields (~ 1.7 mol/mol for H2, 1.38 mol/mol for ethanol) and significantly reduce by-products formation such as acetate or pyruvate by confirming the activation of PP pathway. The highest achieved co-production yield corresponds to ~85 % energy recovery from glucose. Finally, in this study, we would like to discuss the possibility of operating PP pathway under anaerobic condition and its potential for further applicable studies. |
