| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 가을 (10/30 ~ 11/01, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
23권 2호 |
| 발표분야 |
포스터_생체재료 |
| 제목 |
Linker-free antibody conjugation for hydrogel microparticle-based multiplex immunoassay |
| 초록 |
As demands for detecting multiple proteins in a single sample have increased, encoded hydrogel microparticle-based multiplex immunoassay have gained much attention because of its high sensitivity and multiplex capacity rather than the enzyme-linked immunosorbent assay(ELISA). Previously, theseparticles were functionalized with linker(SH-PEG-NHS)-conjugated antibody after particle synthesis via stop flow lithography. However, the linker can damage antigen-binding fragment(Fab) region in antibodies and is vulnerable to hydrolysis in aqueous solution, which limits assay capability. Herein, we present a multiplex immunoassay platform that uses linker-free antibody, or half antibody produced by reduction of whole antibodies which has free thiols that can bond with double bonds in microparticles directly. |
| 저자 |
이현지1, 김주연1, 노윤호1, 김선민2, 문석준1, 봉기완1
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| 소속 |
1고려대, 2보라매병원 |
| 키워드 |
Immunoassay; hydrogel microparticles; linker-free antibody
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| E-Mail |
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