| 초록 |
The major issues, associated with non-viral vectors for small interfering RNAs(siRNAs) are their poor stability and lack of tumor targetability in vivo. In this study, we developed gold-decorated polyethyleneimine (PEI)/siRNA complexes with a corona of PEGylated hyaluronic acid (GDCs). When GDCs were exposed to 50% serum, no significant change in the structural integrity of siRNA was observed at least for 24 h, during which free siRNAs completely degraded. Both GUCs and gold-undecorated (PEI)/siRNA complexes (GUCs) were efficiently internalized into CD44+ melanoma cells (B16F10), but were rarely taken up by CD44- normal fibroblast cells (NIH3T3). For Red fluorescence protein (RFP)-expressing B16F10 cells, GDCs showed the highest gene silencing efficacy, compared to GUCs and free siRNA. Based on their high stability and tumor targetability, GDCs might have promising potential as the siRNA delivery system for cancer therapy. |
