| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 가을 (11/03 ~ 11/05, 대구 엑스코(EXCO)) |
| 권호 |
25권 2호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Selection and Screening of Alpha-Ketoglutaric Semialdehyde Dehydrogenase for Enhancing the 3-Hydroxypropionic acid Production |
| 초록 |
3-Hydroxypropionic acid (3-HP) is one of the key building blocks which is a precursor of value-added chemicals and can be used as an additive or preservative agent in the food industry. The molecule can be produced by a simple biological pathway: dehydration of glycerol to 3-hydroxypropanal (3-HPA) by glycerol dehydratase and then oxidation to 3-HP by aldehyde dehydrogenase. Despite successful production of 3-HP through this pathway, several problems including the toxicity of the 3-HPA hamper the 3-HP production by industrial permissive level. We have made a large-size library that 5 residues were mutated simultaneously for isolating better mutants with respect to catalytic activity toward 3-HPA than previous KGSADH mutants. We isolated the mutant which has 4.65-fold higher activity toward 3-HPA than wild-type. In particular, the recombinant P. denitrificans strain harboring the mutant exhibited 1.3-fold higher 3-HP titer and specific activity than that with the wild-type. |
| 저자 |
박예섭1, Nasir Abdul1, 유태현1, 정규열2, 박성훈3
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| 소속 |
1아주대, 2POSTECH, 3UNIST |
| 키워드 |
Directed Evolution; 3-Hydroxypropionic acid; Aldehyde dehydrogenase
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| E-Mail |
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