| 학회 |
한국공업화학회 |
| 학술대회 |
2018년 가을 (10/31 ~ 11/02, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
22권 2호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Directed evolution of the 3-hydroxypropionic acid production pathway by engineering aldehyde dehydrogenase using an artificial selection device |
| 초록 |
3-Hydroxypropionic acid (3-HP) can be used as a feedstock for production of polymer and chemical. Despite the successful attempts of 3-HP production from glycerol, the key enzymes has low activity. To apply the directed evolutionary approach, we constructed a synthetic selection device using a 3-HP-responsive transcription factor and developed a selection approach for screening 3-HP-producing microorganisms. The method was applied to an aldehyde-binding site library of alpha-ketoglutaric semialdehyde dehydrogenase (KGSADH). Only two serial cultures resulted in enrichment of strains showing increased 3-HP production with a 2.79-fold higher catalytic efficiency toward its aldehyde substrate than the wild-type one. This approach will provide the simple and efficient tool to engineer the pathway enzymes in metabolic engineering. |
| 저자 |
강채원1, 정규열1, 석주연1, 양진아2, 최상진3, 임현규1, 최은종3, 김경진4, 박성훈5, 유태현3
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| 소속 |
1포항공과대, 2서울대, 3아주대, 4경북대, 5유니스트 |
| 키워드 |
3-Hydroxypropionic acid; Directed evolution; Transcription factor; Synthetic biology
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| E-Mail |
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