| 초록 |
Among the processes of the synthesis of silk fibroin hydrogels, enzymatic crosslinking is less toxic than chemical crosslinking, and the reaction proceeds under mild conditions. Horseradish peroxidase (HRP)-mediated crosslinking is the most common method. HRP catalyzes the bridging of phenol groups by radical polymerization through forming radicals using H2O2. Chitosan is a polysaccharide with free amine groups. Chitosan is a representative material with antibacterial properties due to protonated amine group. In this experiment, we tried to make a silk fibroin/chitosan hydrogel through HRP-mediated crosslinking. Chitosan should be modified by 4-hydroxyphenylacetic acid for HRP crosslinking. After the silk fibroin/phenolated chitosan hydrogel was prepared, physical crosslinks of silk fibroin were formed by adjusting the pH(SF/CS-Ph_pH2). These hydrogels had flexible properties than SF/CS-Ph hydrogels by ethanol treatment. The various properties of the hydrogels were assessed. |
