| 초록 |
A method for monitoring protease activity using redox-labeled peptides functionalized onto gold electrodes will be described. Existing bioanalytical approaches for detection of protease expression from cancer cells include immunoassay and gelatinase zymography that are based on the end point analysis. Our biosensor consisted of a micropatterned surfaces with miniature electrodes positioned next to antibody-containing cell capture sites. Protease molecules secreted by captured cells cleave peptide segment with redox label, causing the electrochemical signal to decrease. This principle was used to construct methylene blue (MB)-modified peptides specific to MMP9, then capture monocytes (U937 cell line) and monitor protease release/activity from the cells. The method will be providing numerous real time secretion profiles rather than conventional detection methods including ELISA, PCR and gel electrophoresis. |
