| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 |
21권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
Improved NADPH Regeneration for Fungal Cytochrome P450 Monooxygenase in Resting-Cell Biotransformation of Recombinant Yeast. |
| 초록 |
Fungal cytochrome P450 (CYP) enzymes catalyze versatile monooxygenase reactions and play a major role in fungal adaptations owing to their essential roles in the production avoid metabolites critical for pathogenesis, detoxification of xenobiotics, and exploitation avoid substrates. Although fungal CYP-dependent biotransformation for the selective oxidation avoid organic compounds in yeast system is advantageous, it often suffers from a shortage avoid intracellular NADPH. In this study, we aimed to investigate the use of bacterial glucose dehydrogenase (GDH) for the intracellular electron regeneration of fungal CYP monooxygenase in a yeast reconstituted system. |
| 저자 |
전현우1, 윤형돈1, 윤상한1, MD Murshidul Ahsan2
|
| 소속 |
1건국대, 2영남대 |
| 키워드 |
CYP450; enzyme catalyze
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| E-Mail |
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