| 초록 |
Although giant unilamellar vesicles (GUVs) are useful for a variety of lipid membrane studies, their intrinsically instable membrane properties hamper much wider applications. In this work, we fabricate highly stable GUVs by molecular assembly of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) with newly synthesized amphiphilic triblock copolymers, poly(ethylene oxide)-b-poly(ɛ-caprolactone)-b-poly(ethylene oxide) (PEO-b-PCL-b-PEO) and poly(ethylene oxide)-b-polydimethylsiloxane-b-poly(ethylene oxide) (PEO-b-PDMS-b-PEO), in which the conventional electroformation technique is employed. Subsequently, the mechanical stability of polymer/lipid hybrid bilayers is confirmed by remarkable increases in their membrane modulus. To show the practical applicability of the stable GUVs obtained in this study, we evaluate the ion exchange kinetics through the ion channels in the GUV membrane. |
