| 학회 |
한국고분자학회 |
| 학술대회 |
2014년 봄 (04/10 ~ 04/11, 대전 컨벤션센터) |
| 권호 |
39권 1호 |
| 발표분야 |
의료용 고분자 부문위원회(초청-영어발표/포스터-국문발표 가능) |
| 제목 |
Fluorescence enhancement of DNA-templated silver nanoclusters for nanomolar silver ion detection and its mechanism study |
| 초록 |
A rapid, homogeneous, and in-situ labelable format design to detect silver ion is presented. Fluorescent silver nanoclusters (AgNCs) produced following hydride-mediated reduction of silver ion bound to a cytosine-rich oligodeoxynucleotide (C-rich DNA) was employed as probes for silver ion sensor. Also the sensing mechanism based on the fluorescence enhancement of AgNCs through dimerization of both DNA is elucidated by mass spectrometry, circular dichroism and DNA hybridization analysis. The silver ion detection system is developed by platform of fluorescence “turn-on” assay. The quantum yield of silver ion detected DNA-AgNCs probe is calculated as 43.9 % relative to Rhodamine 6G. Also utilizing the fluorescence enhancement of DNA-AgNCs by silver ions addition, we suggest a quantitative silver ion detection method which has low detection limit as 10 nM. |
| 저자 |
이지현, 김원종
|
| 소속 |
포항공과대 |
| 키워드 |
DNA silver nanoclusters; DNA-AgNCs; Silver ion detection
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| E-Mail |
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