| 학회 |
한국재료학회 |
| 학술대회 |
2016년 가을 (11/16 ~ 11/18, 경주 현대호텔) |
| 권호 |
22권 2호 |
| 발표분야 |
B. 나노화학/바이오 재료 분과 |
| 제목 |
Well-oriented Antibodies on Ultraclean Single-crystalline Au Nanoplate for Attomolar Detection of CRP. |
| 초록 |
Nanosensors for high sensitive C-reactive protein test have been widely studied to predict the risk of cardiovascular diseases. Here, we suggest the combination of cysteine tagged protein G which immobilizes anti-CRPs with correct orientations and an ultraclean and single-crystalline Au nanoplates for the ultrasensitive CRP detection. And we confirmed a high surface coverage of anti-CRPs supported by protein G on the Au nanoplates from atomic force microscopy measurement. Interestingly, the well-ordered anti-CRPs by combination of the Au nanoplate and protein G show the great selectivity toward non-target compounds at their concentration commonly found in blood. Moreover, surface enhanced Raman scattering sensor prepared by the assembly of [Au nanoplate + protein G] complex and Au nanoparticles can detect CRP with 1 aM of detection limit. We expect that the detection platform employing the complex of single-crystalline Au nanoplates and protein G can be used in various high-sensitive biosensor applications which employ the IgG typed antibodies. |
| 저자 |
Ahreum Hwang1, Taejoon Kang2, Bongsoo Kim3
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| 소속 |
1KAIST, 2KRIBB, 3한국생명공학(연) |
| 키워드 |
Au nanoplate; Protein G; CRP; Au nanoparticle; SERS; Biosensor
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| E-Mail |
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