| 초록 |
To overcome a big drawback for cell infiltration and proliferation of nanofiber compared with three dimensional scaffold prepared from other fabricating methods. In this study, the pore size of nanofibers was enlarged by a sonication method in an aqueous or aqueous gelatin solution. Time-dependent/sonicating power-dependent pore sizes, morphological changes, and cell infiltration potentials were evaluated on a series of nanofibers. To evaluate the cell filtration on the scaffold, NIH3T3 cells were seeded and cultured for 1 day, and the levels of penetrated cells into the inner part scaffold were monitored using the confocal laser scanning microscope (CLSM). Cell infilitration potential was much increased with increase of pore size and porosity which were observed by a confocal laser scanning microscopy. In conclusion, sonication method may be very useful to making optimized structured nanofibrous scaffold for tissue regeneration. |
