| 초록 |
Nowdays, methanol is attractive candidate for a carbon feedstock. In contrast with sugar, methanol is not only non-food feedstock, but also price is lower than glucose. In this study, we constructed metabolically engineered Methylobacterium extorquens AM1 well known methylotroph for producing industrial chemicals in various study as a whole cell biocatalyst to produce 1,2-propanediol (1,2-PDO) from methanol. 1,2-PDO is building blocks in plastics industry, in de-icing and anti-freeze fluids, and as additive in pharmaceuticals, laundry detergent, cosmetics, and so on. To produce 1,2-PDO, heterogenous genes for 1,2-PDO biosynthesis pathway were introduced in M. extorquens AM1. Firstly, dihydroxyacetone phosphate (DHAP) was converted to methylglyoxal by mgsA (methylglyoxal synthase). In sequence methylglyoxal was converted to hydroxyacetone by yqhD (NADP-dependent alcohol dehydrogenase). Lastly, 1,2-PDO was produced from hydroxyactone by gldA (glycerol dehydrogenase). We confirmed constructed biosynthetic pathway working properly by enzyme assay. Finally, biotransformation of methanol to 1,2-PDO was verified in flask scale. |
