| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 봄 (05/01 ~ 05/03, 부산 벡스코(BEXCO)) |
| 권호 |
23권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Microfluidic high-throughput screening of model alga, Chlamydomonas reinhardtii with superior photosynthetic productivity using phototaxis |
| 초록 |
Photosynthetic organisms evolved phototaxis to find optimal light condition for photosynthesis. Here we report high-throughput screening system using phototaxis of Chlamydomonas reinhardtii, for rapid isolation of strains with enhanced photosynthetic efficiencies in microfluidic system. We proved the correlation between phototaxis and PSII efficiency by quantitative analysis of phototaxis. We enriched the strains with improved PSII efficiency by isolating cells showing fast phototactic response from 10,000 mutants. Among 147 strains isolated after screening, 94.6% showed enhanced growth over the parental strain. Two mutants showed much improved performances with up to 1.9- and 8.1-fold increases in growth and lipid production, respectively. Our approach provides a powerful screening tool for rapid improvement of microalgae to enhance photosynthetic efficiency. |
| 저자 |
최진원1, 곽호석2, 심상준1
|
| 소속 |
1고려대, 2동양미래대 |
| 키워드 |
Microfluidic; high-throughput; Chlamydomonas reinhardtii; phototaxis
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| E-Mail |
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