| 초록 |
D-xylonic acid, one of the top 30 high-value chemicals by the US Department of Energy (DOE), has various applications, such as substrate for biosynthesis of 1,2,4-butanetriol, concrete additive, and pH regulator for food, chemical, pharmaceutical industries. To date, D-xylonic acid has been produced by microbial conversion of D-xylose, the hemicellulosic pentose sugar. To acquire D-xylose from hemicellulose, however, additional process of enzymatic hydrolysis is required, and it can significantly increase the cost and time for whole process. Here, we engineered Corynebacterium glutamicum for the production of D-xylonic acid directly from hemicellulose. First, xylose dehydrogenase from Caulobacter crescentus was introduced for the D-xylonic acid production. Next, xylose transporter was introduced for the enhanced D-xylose uptake. Finally, a xylan degradation system was also added to degrade hemicellulose into D-xylose for the direct production of D-xylonic acid from hemicellulose. To the best of our knowledge, this is the first report of developing consolidated bioprocess for the production of D-xylonic acid. |
