| 학회 |
한국공업화학회 |
| 학술대회 |
2015년 가을 (11/04 ~ 11/06, 제주국제컨벤션센터(ICCJEJU)) |
| 권호 |
19권 2호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Stabilization of Carbonic Anhydrase for Efficient Capture of Carbon Dioxide via Corynebacterium Cell Display |
| 초록 |
Mineral carbonation is the most effective carbon capture technique, but the reaction is too slow for industrial applications. Therefore, highly efficient biocatalyst, carbonic anhydrases (CAs), have been widely studied for CO2 capture. Unfortunately, their practical uses are restricted by their poor stability and high cost. Here we introduced a new biological platform for the stabilization of CA using cell display. Neisseria gonorrhoeae CA (ngCA) was displayed on the mycolic layer of Corynebacterium glutamicum via genetic modification using an anchoring protein porinB with a triplet of a G4S linker. This whole-cell catalyst maintained the activity to 60 % after 24 h of the incubation at 60 oC, while the activity of commercial CA dropped to below 10 %. We expect that Corynebacterium display is a promising approach to achieve a thermally stable and economic enzymatic system for CO2 capture. |
| 저자 |
남윤성1, 정기준2, 구본일3, 최재웅2, 송석영3
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| 소속 |
1KAIST 신소재공학과 / KAIST Institute for NanoCentury (KINC CNiT), 2KAIST 생명화학공학과, 3KAIST 신소재공학과 |
| 키워드 |
carbon capture; corynebacterium; cell display; carbonic anhydrase; thermostability |
| E-Mail |
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