| 학회 |
한국화학공학회 |
| 학술대회 |
2017년 가을 (10/25 ~ 10/27, 대전컨벤션센터) |
| 권호 |
23권 2호, p.1710 |
| 발표분야 |
생물화공 |
| 제목 |
Metabolic engineering of Escherichia coli for the production of Poly(2-hydroxyisovalerate-co-lactate) |
| 초록 |
Polyhydroxyalkanoates (PHAs) containing 2-hydroxyacids such as lactate (LA) and 2-hydroxybutyrate (2HB) have recently been produced by metabolically engineered microorganisms. Here, we further engineered E.coli to produce PHAs containing 2-hydroxyisovalerate (2HIV). To produce PHA containing only 2HIV and LA monomers, genes encoding enzymes involved in competing pathways for pyruvate generation are deleted. Culturing of this engineered E. coli strain produced poly(20 mol% 2HIV-co-80 mol% LA) to the polymer content of 9.6% w/w. [This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science, ICT through the National Research Foundation (NRF) of Korea (NRF-2012M1A2A 2026556 and NRF-2012M1A2A2026557) and NRF grant funded by the MSIP (NRF-2016R1A2B4008707)] |
| 저자 |
이영준1, 양정은1, 김제웅1, 오영훈2, 최소영1, 이 혁2, 박아름2, 신지훈2, 박시재3, 이상엽1
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| 소속 |
1KAIST, 2한국화학(연), 3이화여자대 |
| 키워드 |
생물화공 |
| E-Mail |
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| 원문파일 |
초록 보기 |
