| 초록 |
Angiogenesis is an essential process in tissue regeneration. In this study, we prepared a suspended fibroblast-derived matrix (sFDM) and examined its angiogenic potential. An ointment type hydrogel composed of Pluronic F127 and hyaluronic acid (HA) was used for effectively delivering sFDM into injured site. The sFDM-included Pluronic/HA (PH/sFDM) was characterized via SEM imaging, rheological analysis, and biochemical proteins array. In vitro study demonstrated that endothelial cells cultured with sFDM presented a higher cell viability and monocytes also showed a high level of growth factor secretion. In addition, we evaluated a therapeutic effect of our sFDM for angiogenesis via two different in vivo models. In the wound model, PH/sFDM group revealed an effective wound closure and histological recovery. The ischemia model also demonstrated that PH/sFDM group has a strong blood reperfusion and reduced muscle fibrosis. Our sFDM shows a great potential toward therapeutic angiogenesis. |
