| 학회 |
한국화학공학회 |
| 학술대회 |
2019년 봄 (04/24 ~ 04/26, 제주국제컨벤션센터) |
| 권호 |
25권 1호, p.369 |
| 발표분야 |
생물화공(Biochemical Engineering) |
| 제목 |
Structural Characterization of PETase toward Superior Poly(ethylene terephthalate) Degradation |
| 초록 |
Plastics, including poly(ethylene terephthalate) (PET), are very popular with their desirable characteristics. However, non-biodegradability, once thought to be great merit of plastics, causes the severe environmental problem. With the recently found PET-degrading bacterium, Ideonella sakaiensis, its possible use in microbial PET degradation becomes considerable. Here we firstly report the crystal structure of I. sakaiensis PETase (IsPETase) with high resolution. It helps to confirm the catalytic features of IsPETase and predict its molecular mechanism on degrading PET. Moreover, we succeed in developing the variant of IsPETase with 32.4% enhanced PET-degradability over its wild type using structural-based protein engineering. In this study, the reasons IsPETase has superior activity were successfully predicted with the 3D structure and related biochemical studies. This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries (NRF-2012M1A2A2026556 and NRF-2012M1A2A2026557) from the Ministry of Science and ICT through the National Research Foundation of Korea |
| 저자 |
조인진1, 주성준2, 서호균2, 김경진2, 이상엽1
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| 소속 |
1한국과학기술원(KAIST), 2경북대 |
| 키워드 |
생물화학공학 |
| E-Mail |
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| 원문파일 |
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