| 초록 |
This study makes to reduce a protein adsorption to the surface and obtain a high-yield concentration while we carry out the separation and extraction of protein. Adsorption of Protein, the most representative material among biomolecules, occurs at surface of membrane and disturbs efficient separation and extraction. To prevent the adsorption of protein, surface of membrane was modified into hydrophilic surface. In order to obtain hydrophilic surface, we used hydrophilic polysaccharide such as Pectin, Dextran, Hyaluronic acid and chitosan which is known a effective biomolecule's adsorption preventer. The modified surface of membrane was observed by AFM and adsorption of protein was measured by BCA assay. As a result, concentration of BCA rises and adsorbed BCA in surface of membrane is reduced. Among polysaccharides, membrane coatede by hyaluronic acid showed the best adsorption prevention effect. |
