| 초록 |
In this study, we report the functional surface display of laccase, cotA of B. subtilis, which could be applied to decompose industrial dyes. CotA spore coat protein is known to have multi-copper oxidase (laccase) activity. First, we confirmed the decolorization rate of Acid green 25, anthraquinone dye by 20 % in 5 hrs using a wild-type Bacillus subtilis spore which containing original copy of CotA. To express CotA protein on the surface of the spores, anchoring motives CotE was used. And we confirmed improved decolorization rate of Acid green 25 by 50% in 5 hrs using a recombinant Bacillus subtilis spore. His6-tag was added at the C-terminal of target protein, CotA. Exact expression of CotE-CotA fusion protein was confirmed by SDS-PAGE and Western analysis of spore extracted protein. The spore surface expression of fusion, CotE-CotA, was confirmed by flow cytometry using FITC labelled anti-His6 antibody. |
