| 초록 |
Liver fibrosis is an important health issue for which there are no effective medicine, leading to liver cirrhosis and cancer. Cell therapy based on mesenchymal stem cells (MSCs) is one of attractive options. However, the limitation of cell transplantation is rest. In order to overcome it, we designed MSCs derived exosome-based therapy. To isolate exosome, human adipose-derived mesenchymal stem cells (ADSCs) were cultured for 24h in conditioned media. Exosome was purified using a Tangential Flow Filtration System. The expression level of α-SMA was remarkably decreased depending on the treatment concentration of A-Exo at hepatic stellate cells. Exosome treatment significantly decreased expression mRNA of pro-fibrogenic marker. In this study, we investigate the potential of stem cell-derived exosome as the new therapeutic approach for liver fibrosis treatment. The beneficial effect of A-exo were confirmed in vitro and in vivo tests. |
