| 학회 |
한국화학공학회 |
| 학술대회 |
2014년 봄 (04/23 ~ 04/25, 창원컨벤션센터) |
| 권호 |
20권 1호, p.340 |
| 발표분야 |
생물화공 |
| 제목 |
Hyperthermophilic Carbonic Anhydrase: Application for CO2 Capture System Development |
| 초록 |
In order to employ carbonic anhydrases (CAs, potential biocatalysts for CO2 sequestration) under the non-natural process conditions, highly-thermostable CAs should be explored. Here, we expressed the codon-optimized sequence of PMCA, cloned from the thermophile Persephonella marina EX-H1 found in marine vents, and characterized its hyper-thermostable properties. Removal of the PMCA signal peptide (sp) resulted in the production of about 5 times more purified protein, PMCA(sp-), than from the intact gene in an E. coli expression system. PMCA(sp-) has a wide pH tolerance (optimum: pH 7.5), retained 80% of its activity after 15 min incubation at 100°C and almost 50% activity even after 2 h at 100°C. Apparent Km and Vmax for the p-nitrophenylacetate were 5.90 mM and 0.020 µmol min-1. Various metal ions were examined to enhance or inhibit activity. Finally, we demonstrated that in the presence of Ca2+, PMCA(sp-) readily catalyzed the conversion of CO2 to CaCO3 (calcite form). |
| 저자 |
백승필, 민기하, 유영하, 손려강, 여기백, 박기성, 전소영, 기미란
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| 소속 |
고려대 |
| 키워드 |
Carbonic Anhydrase; CO2 Capture; Hyperthermophilic
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| E-Mail |
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| VOD |
VOD 보기 |
| 원문파일 |
초록 보기 |
