| 학회 |
한국화학공학회 |
| 학술대회 |
2017년 봄 (04/26 ~ 04/28, ICC 제주) |
| 권호 |
23권 1호, p.474 |
| 발표분야 |
생물화공 |
| 제목 |
One-step fermentative production of poly(lactate-co-glycolate) by metabolically engineered Escherichia coli |
| 초록 |
Poly(lactate-co-glycolate) (PLGA) is a widely used biodegradable and biocompatible synthetic polymer. Here we report one-step fermentative production of PLGA in engineered Escherichia coli harboring an evolved polyhydroxyalkanoate (PHA) synthase that polymerizes D-lactyl-CoA and glycolyl-CoA into PLGA. Introduction of the Dahms pathway enables production of glycolate from xylose. An evolved propionyl-CoA transferase converts D-lactate and glycolate to D-lactyl-CoA and glycolyl-CoA. Deletion of adhE, frdB, pflB and poxB prevents byproduct formation. We also demonstrate modulation of the monomer fractions in PLGA, either increasing the proportion of D-lactate by overexpressing ldhA and deleting dld, or increasing the proportion of glycolate by deleting aceB and glcDEFGB.[This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science, ICT and Future Planning (MSIP) through the National Research Foundation (NRF) of Korea (NRF-2012M1A2A2026556 and NRF-2012M1A2A2026557)]. |
| 저자 |
김동인1, 최소영1, 박시재2, 김원준1, 양정은1, 이 혁3, 신지훈3, 이상엽1
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| 소속 |
1한국과학기술원, 2명지대, 3한국화학(연) |
| 키워드 |
생물화공 |
| E-Mail |
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| 원문파일 |
초록 보기 |
