| 학회 |
한국화학공학회 |
| 학술대회 |
2011년 가을 (10/26 ~ 10/28, 송도컨벤시아) |
| 권호 |
17권 2호, p.1539 |
| 발표분야 |
생물화공 |
| 제목 |
Investigation on Bulk-scale Adhesive Force of Recombinant Mussel Adhesive fp-3 |
| 초록 |
Mussel adhesive proteins (MAPs) have received increased attention as potential biomedical and environmentally friendly underwater adhesives. Particularly, foot protein type 3 (fp-3), is located at the interface between the substratum and the adhesion plaque of mussels, and it is expected as one of the important components for surface adhesion with high concentration of 3, 4-dihydroxyphenyl-L-alanine (DOPA). Although our previous research reported the production of Mytilus galloprovincialis fp-3 variant A (fp-3A) in Escherichia coli, its biochemical characterization and practical use were difficult because of its low productivity. In this work, fp-3A gene was codon-optimized based on the preference of E. coli codon usage and signal sequence was eliminated. Mass production of recombinant fp-3 fused with a hexahistidine affinity ligand and its purification with affinity chromatography were successed with ~47 mg/L yield. Bulk-scale adhesive forces of purified recombinant fp-3 were measured without and with in vitro DOPA modification. Collectively, we can expect that recombinant fp-3A may be used as a practical bulk-scale bioadhesive. |
| 저자 |
양병선1, 최유성2, 강동균1, 차형준3
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| 소속 |
1Department of Chemical Engineering, 2POSTECH, 3Chungnam National Univ. |
| 키워드 |
mussel adhesive protein; bulk adhesion
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| E-Mail |
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| 원문파일 |
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