| 초록 |
The immobilization of protein on solid surfaces is a key step in the development of medical diagnostics, biomaterials, and cell and tissue engineering as well as biochips and biosensors. The functional protein assemblies are receiving broad scientific attention as simple and flexible model system in bioelectronics. However, the research about the signal transfer of protein within the assemblies was not much reported because they had very complex structure. In this study, the immobilization of cytochrome c on the silver nanoparticles in the TiO2 gel film was reported. An ultrathin TiO2 gel film that contains silver ions was prepared on a quartz plate by doping Ag+ ions into TiO2 gel matrix. And then cytochrome c was immobilized on the silver nanoparticles in the TiO2 gel film. The formation of the silver nanoparticles formation and the immobilization of cytochrome c were characterized by quartz crystal microbalance (QCM), UV-vis spectroscopy, AFM, XPS, and potentiostat. |
