| 학회 |
한국고분자학회 |
| 학술대회 |
2007년 가을 (10/11 ~ 10/12, 일산킨텍스) |
| 권호 |
32권 2호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
Thermosensitive Hyaluronic Acid/Pluronic F127/Microsphere Composite Hydrogel for Cartilage Tissue Engineering |
| 초록 |
Three-dimensional hydrogel is a useful cell carrier for injectable implantation. Hyaluronic acid (HA) is an important component of the extracellular matrix (ECM) of connective tissues. F127 Hydrogel which displays temperature responsiveness and non-toxicity is a good candidate for drug-delivery systems. In this study, we hypothesize that HA/F127/microsphere composite hydrogel may have some advantages in the cartilage formation. For in vitro and in vivo tests, chondrocytes were encapsulated in the hydrogel, mixed with microspheres. The cell-incorporated hydrogels were cultured in 24-well plates for two weeks and then implanted into nude mouse subcutaneously. The composite constructs were evaluated using Safranin O staining for GAG. Cartilage-specific gene expression was also determined using RT-PCR. This composite hydrogel system is expected to be useful as an injectable hydrogel for cartilage regeneration. |
| 저자 |
배순언1, 손준식1, 박귀덕1, 신흥수2, 안광덕1, 한동근1
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| 소속 |
1한국과학기술(연), 2한양대 |
| 키워드 |
Pluronic F127; Hyaluronic acid; Thermosensitive hydrogel; Microsphere; Chondrocyte
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| E-Mail |
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