| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 가을 (11/03 ~ 11/05, 대구 엑스코(EXCO)) |
| 권호 |
25권 2호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Precise rebalancing of precursors by fine -control of gapA expression for isoprenoid biosynthesis in E. coli |
| 초록 |
Isoprenoids form one of the most diverse group of natural products. Isoprenoids biosynthesis via the DXP pathway requires equimolar G-3-P and pyruvate to divert carbon flux toward the products. In this study, we demonstrated that precursor rebalancing is one of the critical steps for the isoprenoids biosynthesis in Escherichia coli via the DXP pathway. First, we constructed a lycopene-overproducing E. coli strain by the amplification of the native DXP pathway using synthetic promoters and redesigned 5’-UTR. Second, fine-controlled precursor balancing was investigated by tuning PpsA or GAPDH. The results showed that tuning-down of gapA improved the specific lycopene by 45% compared to the overexpression of ppsA. The specific lycopene content in the strains with down-regulated gapA increased by 97% compared to that in the parental strain. Our results indicate that gapA is the best target for precursor balancing to increase biosynthesis of isoprenoids. |
| 저자 |
정금영, 정규열, 석주연, 정주영, 임재형
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| 소속 |
포항공과대 |
| 키워드 |
isoprenoid; gapA; precise-balancing; fine-control; E. coli;
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| E-Mail |
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