| 학회 |
한국화학공학회 |
| 학술대회 |
2019년 가을 (10/23 ~ 10/25, 대전컨벤션센터) |
| 권호 |
25권 2호, p.1479 |
| 발표분야 |
생물화공 (Biochemical Engineering) |
| 제목 |
Unraveling Structure and Function of PETase toward Superior Poly(ethylene terephthalate) Degradation |
| 초록 |
Poly(ethylene terephthalate) (PET) are very popular plastics, but serious contaminants with its non-biodegradability. Therefore, bio-based PET waste degradation becomes significant. With the recently found PET-degrading bacterium, Ideonella sakaiensis, its possible use in microbial PET degradation becomes considerable. Here we firstly report the crystal structure of I. sakaiensis PETase (IsPETase) with high resolution. It helps to confirm the catalytic features of IsPETase and predict its molecular mechanism on degrading PET to understand the essential factors to degrade PET efficiently. Moreover, we succeed in developing the variant of IsPETase with 32.4% enhanced PET-degradability over its wild type using structural-based protein engineering. This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science and ICT (MSIT) through the National Research Foundation (NRF) of Korea (NRF-2012M1A2A2026556 and NRF-2012M1A2A2026557). |
| 저자 |
조인진1, 주성준2, 서호균2, 손현철2, 사공혜영2, 신태주3, 최소영1, 김경진2, 이상엽1
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| 소속 |
1한국과학기술원(KAIST), 2경북대, 3울산과학기술원(UNIST) |
| 키워드 |
생물화학공학 |
| E-Mail |
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| 원문파일 |
초록 보기 |
