| 학회 |
한국화학공학회 |
| 학술대회 |
2019년 가을 (10/23 ~ 10/25, 대전컨벤션센터) |
| 권호 |
25권 2호, p.1478 |
| 발표분야 |
생물화공 (Biochemical Engineering) |
| 제목 |
Highly Efficient PET Degradation by Rational Protein Engineering of Thermo-Stable PETase from Ideonella sakaiensis |
| 초록 |
As a solution for Poly(ethylene terephthalate) (PET) waste problem, microbial PET degradation in normal temperature became considerable thanks to Ideonella sakaiensis PETase (IsPETase). However, its low thermal stability limits its ability for efficient and practical enzymatic degradation of PET. Using the structural information on IsPETase, we developed a rational protein engineering strategy for high thermal stability to improve PET degradability. In particular, the IsPETaseS121E/D186H/R280A variant, which was designed to have a stabilized β6-β7 connecting loop and extended subsite IIc, showed enhanced PET degradability by 14-fold at 40 °C compared to native IsPETase using in vitro system. The designed structural modifications were further verified through structure determination of the variant, and this variant showed highly efficient PET degradation through in vivo system as well. This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science and ICT through the National Research Foundation (NRF) of Korea (NRF-2012M1A2A2026556 and NRF-2012M1A2A2026557) |
| 저자 |
조인진1, 손현철2, 주성준2, 서호균2, 사공혜영2, 최소영1, 김경진2, 이상엽1
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| 소속 |
1한국과학기술원(KAIST), 2경북대 |
| 키워드 |
생물화학공학 |
| E-Mail |
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| 원문파일 |
초록 보기 |
