| 학회 |
한국고분자학회 |
| 학술대회 |
2011년 봄 (04/07 ~ 04/08, 대전컨벤션센터) |
| 권호 |
36권 1호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
Intervertebral Disc Regeneration Using Annulus Fibrosus Cellon Purified Alginate Sponge |
| 초록 |
The application of annulus fibrosus (AF) cells is promising therapeutic approach for intervertebral disc regeneration. In this study, purified alginate sponge were used in intervertebral disc regeneration. AF cells were seeded in purified and non-purified alginate sponge, and then cell viability analyzed by MTT assay after 3, 7, 10 and 14 days. RT-PCR was assessed to measure mRNA expression for type I, II collagen and aggrecan from AF cells in purified and non-purified alginate sponge. The alginate sponge evaluated by histology through H&E, Safranin-O and MTS staining in vivo. From the results of the RT-PCR and histology staining characteristic of AF cell in the purified alginate sponge reduced better than non-purified alginate sponge. This result indicates that purified alginate sponge is useful for intervertebral disc regeneration. This research was supported by WCU (R31-20029) and XRC (A040004). |
| 저자 |
황지혜1, 배지연1, 김민정1, 송정은1, 윤건호2, 이동원1, 강길선1
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| 소속 |
1전북대, 2가톨릭대 |
| 키워드 |
Disc; Annulus fibrosus; Alginate; Sponge
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| E-Mail |
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