| 초록 |
Bacterial microcompartment (BMC) has attractive properties for application. In this study, two anaerobic enzymes, trans-2-enoyl-CoA reductase (Ter) and [FeFe] hydrogenase from C. acetobutylicum ATCC 824 were encapsulated in pdu BMC and their activity in BMC was verified. With the encapsulated [FeFe] hydrogenase, H2 was produced in aerobic condition by the encapsulated [FeFe] hydrogenase. To enhance the H2 production rate, pocR, a pdu BMC assembly helper protein from Citrobacter freundii was introduced additionally and 43.9±9.3 μmol gDCW-1 of H2 production rate in aerobic condition was achieved. Also, the production of butyric acid increased more than twice compared to the control strain by the encapsulation of Ter in pdu BMC. To identify the relation of enzyme expression level and signal peptide with encapsulation, fluorescent protein was encapsulated and fluorescent patterns were observed by confocal microscopy. [This work was supported by the C1 Gas Refinery Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2016M3D3A1A01913250)] |
