| 초록 |
Previously, phenol’s biological production from renewable resources has been limited due to its toxicity to microorganisms. In this study, we simultaneously engineered 18 Escherichia coli strains for the production of phenol by employing the strategy of synthetic regulatory sRNA technology. The 18 engineered E. coli strains showed significant differences in the production of tyrosine, tyrosine phenol-lyase activity, and tolerance to phenol. These results led to much variation in their phenol-producing capabilities. The final concentration of phenol in the glycerol tributyrate phase and fermentation broth reached 9.84 and 0.3 g/L, in 21 h, respectively. This is the highest titer achieved by microbial fermentation. Although further engineering is required to be competitive with the current petro-based process, the strategies used for this study will provide a valuable framework for the microbial production of toxic chemicals. [This work was supported by the Intelligent Synthetic Biology Center through the Global Frontier Project (2011-0031963) funded by the Ministry of Science and ICT (MSIT) through the National Research Foundation (NRF) of Korea.] |
