| 학회 |
한국화학공학회 |
| 학술대회 |
2015년 봄 (04/22 ~ 04/24, 제주 ICC) |
| 권호 |
21권 1호, p.408 |
| 발표분야 |
생물화공 |
| 제목 |
Synthetic small regulatory RNAs-based bacterial knockdown and its role in engineering microbial production of tyrosine and cadaverine |
| 초록 |
A toolkit of synthetic sRNAs for manipulating gene expression was developed in E. coli. The target binding sequence of MicC was inserted to the translation initiation region of genes of interest. Knockdown on a fluorescent protein called DsRed2 showed that MicC scaffold-based synthetic sRNA was able to properly repress DsRed2 expression. Furthermore, by knocking down four candidate genes in 14 different strains, we isolated a tyrosine overproducer with a titer of 2 g/L. With a library of 130 synthetic sRNAs, several knockdown targets were identified, resulting in an increased cadaverine production by 55%. [This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries (NRF-2012M1A2A2026556); the Intelligent Synthetic Biology Center through the Global Frontier Project (2011-0031963) of the Ministry of Education, Science and Technology (MEST) through the National Research Foundation of Korea] |
| 저자 |
Ziwei Luo, 유승민, 나도균, 정해나, 박혜권, 이상엽
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| 소속 |
한국과학기술원 |
| 키워드 |
Tyrosine; cadaverine; Sythetic small regulatory RNA; Escherichia coli
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| E-Mail |
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| 원문파일 |
초록 보기 |
