| 학회 |
한국화학공학회 |
| 학술대회 |
2018년 가을 (10/24 ~ 10/26, 대구 EXCO) |
| 권호 |
24권 2호, p.1462 |
| 발표분야 |
생물화공(Biochemical Engineering) |
| 제목 |
Metabolically Engineered Escherichia coli and Corynebacterium glutamicum for the Production of 1,5-diaminopentane |
| 초록 |
Bio-based production of 1,5-diaminopentane is an alternative to the petroleum-based chemical synthesis. Here, we developed metabolically engineered Escherichia coli and Corynebacterium glutamicum overproducing 1,5-diaminopentane. In E. coli, degradation pathways of 1,5-diaminopentane were inactivated and cadA (lysine decarboxylase) was overexpressed. Chromosomal promoter exchange by trc promoter of dapA gene (dihydrodipicolinate synthase) was done to increase L-lysine biosynthetic pool. The strain produced 9.61 g/L of 1,5-diaminopentane in fed-batch culture. C. glutamicum (U2 strain), is used to overexpress cadA gene. However, 1,5-diaminopentane was not produced and L-lysine was detected in the medium. The cadA gene was then modified by codon adaptation program. C. glutamicum U2 strain overexpressing the optimized cadA produced 31.94 g/L of 1,5-diaminopentane in fed-batch culture. (NRF-2012M1A2A2026556 and NRF-2012M1A2A2026557). |
| 저자 |
Prabowo Cindy Pricilia Surya1, 박석현2, Zhi Gang Qian3, 이상엽1
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| 소속 |
1Metabolic and Biomolecular Engineering National Research Laboratory, 2Department of Chemical and Biomolecular Engineering, 3KAIST |
| 키워드 |
생물화공 |
| E-Mail |
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| 원문파일 |
초록 보기 |
