| 초록 |
3-Hydroxypropionic acid (3-HP) is an important platformchemical which hasdiverse applications in paint, polymer and diaper industries. Itcan be produced from crude glycerol, an abundant byproduct of biodieselproduction, by two enzymatic reactions. Todevelop suitable microbial cell factories, we studied various host strains,genes, pathway, toxicity, cell physiology, etc. for last ~15 years. The workincludes selection of proper host microorganisms, optimization of geneexpression, enzyme engineering, deletion of competing and 3-HP degradation pathways,optimization of energy and cofactor metabolisms, transport, adaptive evolution,etc. Many strains have been developed and transferred to a collaboratingcompany, and the company has been working on bioreactor-scale fermentation,downstream processing and scale-up for commercialization. This lecturessummarizes and discusses our journey with 3-HP. |
