| 학회 |
한국고분자학회 |
| 학술대회 |
2008년 봄 (04/10 ~ 04/11, 컨벤션 뷰로(대전)) |
| 권호 |
33권 1호 |
| 발표분야 |
기능성 고분자 |
| 제목 |
Structure modification of water-soluble conjugated polymers for enhanced sensitivity of FRET-based DNA detection |
| 초록 |
Water-soluble cationic conjugated polymers (CCPs) with two different counterions are studied and compared as a donor in fluorescence resonance energy transfer (FRET) to fluorescein-labeled single-stranded DNA (ssDNA-Fl). Fluorescence quenching of the acceptor (Fl) via photo-induced charge transfer (PCT) in the polymer/ssDNA-Fl electrostatic complex should be minimized to improve DNA detection sensitivity. The energy-wasting PCT can be controlled by simply exchanging counterions accompanied for charge compensation of the CCPs. Large counterions perturb the D-A intermolecular distance in molecular scale, resulting in a substantial decrease of PCT and enhanced FRET-induced Fl signal. Structure modification of CCPs using different counterions can fine-tune the competition between energy transfer and PCT quenching for optimized FRET-based DNA sequence detection. |
| 저자 |
강미정, 우한영
|
| 소속 |
부산대 |
| 키워드 |
water-soluble conjugated polymer; FRET(fluorescence resonance energy transfer); DNA detection
|
| E-Mail |
|
