Silicone rubber is used for a wide variety of biomedical and industrial applications due to its good mechanical properties, combined with a hydrophobic surface. Frequently, however, it is desirable to alter the surface hydrophobicity of silicone rubber. Often this is done by plasma treatments but the effects are usually transient. In this study, surfaces of medical grade silicone rubber have been repeatedly modified by means of oxygen, argon, carbon dioxide, and ammonia RF plasma treatments with a 24 h time interval in between treatments. Treated samples were stored in air prior to surface characterization by water contact angle measurements, X-ray photoelectron spectroscopy (XPS), streaming potential measurements, and profilometry for surface roughness. The carbon percentage of the surfaces decreased after plasma treatment, while the silicon and oxygen percentages increased irrespective of the plasma used. The formation of Si-O-Si bridges between siloxane chains after plasma treatment was demonstrated by the appearance of a new component in the Si-2p peak but the degree to which this occurred differed per gas. Streaming potential measurements in a 10 mM potassium phosphate buffer indicated a more negatively charged surface for treated samples compared to untreated samples (-23.3 mV at pH 7.0). Surface roughness increased slightly for repeatedly plasma-treated samples from R(A) = 0.35 mu m to R(A) = 0.46 mu m, while scanning electron microscopy showed the presence of several ＇cracks＇ spanning the surface after repeated treatment. Argon, carbon dioxide, and ammonia plasmas significantly reduced the advancing water contact angle from 115 degrees to 58 degrees, 72 degrees, and 85 degrees, respectively, on a more permanent basis (especially when the treatments were repeated after recovery). Oxygen plasma effects on water contact angles generally disappeared within 5 h, also after repeated treatment.