Several methods for disintegration of biofuel pellets were tested and compared for their ability to break up the pellets into the original particles of the raw material. Analyses performed on softwood pellets and straw pellets concluded that wet disintegration in water at ambient temperature is insufficient for a determination of the internal particle size distribution of wood-and straw pellets. When the wet disintegration was performed with water heated to the boiling point and coupled with mechanical disintegration in terms of stirring a more complete disintegration of the pellets was obtained. Based on the results obtained in the initial study a round robin was set up including six European laboratories where the selected method was tested. In the round robin test the method combining heated water and stirring of the slurry was tested on solid biofuel pellets produced of comminute straw, deciduous wood and coniferous wood respectively. With the method a satisfactory disintegration was obtained of all three types of pellets. Further wet disintegration of coniferous pellets was compared to a dry disintegration using a hammer mill. The dry disintegration of the coniferous pellets resulted in smaller particle sizes compared to the wet disintegration using heated water and stirring of the slurry indicating a further disintegration of the original particles in the hammer mill process. Overall the wet disintegration combined with mechanical impact was found to be the most suitable method for disintegration of solid biofuel pellets. Combined with sieving analysis the method gives realistic image of the internal particle size distribution of solid biofuel pellets. (C) 2010 Elsevier Ltd. All rights reserved.