An optically accessible single-cylinder high-speed direct-injection (HSDI) diesel engine was used to investigate the spray and combustion processes for biodiesel blends under different injection strategies. The experimental results indicated that the heat release rate was dominated by a premixed combustion pattern and the heat release rate peak became smaller with injection timing retardation. The ignition and heat release rate peak occurred later with increasing biodiesel content. Fuel impingement on the wall was observed for all test conditions. The liquid penetration became longer and the fuel impingement was stronger with the increase of biodiesel content. Early and late injection timings result in lower flame luminosity due to improved mixing with longer ignition delay. For all the injection timings, lower soot luminosity was seen for biodiesel blends than pure diesel fuel. Furthermore, NO(x) emissions were dramatically reduced for premixed combustion mode with retarded post-TDC injection strategies. (C) 2009 Elsevier Ltd. All rights reserved.