In order to assess their accuracy for the determination of glucose during the enzymatic hydrolysis of pretreated lignocellulosic biomass, four different blood glucose monitors (BGMs), each utilizing a different enzymatic mechanism for the determination of glucose, were utilized in an experimental setup, which compares the efficiency of ionic liquid pretreatment with dilute acid and alkaline pretreatments applied on corncob. Among the tested devices, Optium Xceed was found to be the most accurate device for the determination of glucose where Accu-Chek Active was the least accurate BGM, yielding similar results to those obtained with DNS method. Based on the HPLC results, the % error values for Optium Xceed ranged between 3.9-10.5% for the determination of glucose concentration. Upon enzymatic hydrolysis, ionic liquid and alkaline pretreatments gave similar glucose yields, which were slightly higher than the dilute acid pretreatment, which were 31.9%, 31.0% and 27.8%, respectively, based on untreated corncob. (C) 2011 Elsevier Ltd. All rights reserved.