The aim of this work was to investigate the potential conversion of Chlamydomonas reinhardrii biomass harvested after hydrogen production. The spent algal biomass was converted into nitrogen-rich bio-char, biodiesel and pyrolysis oil (bio-oil). The yield of lipids (algal oil), obtained by solvent extraction, was 15 +/- 2% w/w(dry-biomass). This oil was converted into biodiesel with a 8.7 +/- 1% w/w(dry-biomass) yield. The extraction residue was pyrolysed in a fixed bed reactor at 350 degrees C obtaining bio-char as the principal fraction (44 +/- 1% w/w(dry-biomass)) and 28 +/- 2% w/w(dry-biomass) of bio-oil. Pyrolysis fractions were characterized by elemental analysis, while the chemical composition of bio-oil was fully characterized by GC-MS, using various derivatization techniques. Energy outputs resulting from this approach were distributed in hydrogen (40%), biodiesel (12%) and pyrolysis fractions (48%), whereas bio-char was the largest fraction in terms of mass. (C) 2011 Elsevier Ltd. All rights reserved.