Whole cells of Rhodococcus sp. ECU0066 were used a catalyst for resolution of racemic sulfoxides, as an alternative to asymmetric oxidation of sulfides for efficient production of enantiopure sulfoxides. Racemic sulfoxides were excellent substrates for biotransformation because of their lower biotoxicity compared to sulfides. Determination of apparent kinetic parameters indicated that phenyl methyl sulfide (PMS), but not racemic phenyl methyl sulfoxide (rac-PMSO) caused substrate inhibition. (S)-PMSO was formed at a higher concentration and good enantiomeric excess (37.8 mM and 93.7% ee(S)) in a fed-batch reaction, than by an asymmetric oxidation of PMS (10 mM and 80% ee(p) (S)). The bacterium also displayed fairly good activity (yields, 22.7-43.2%; within 1-8 h) and enantioselectivity (ee(S) > 99.0%) towards para-substituted (methyl and chloro) phenyl methyl sulfoxides and ethyl phenyl sulfoxide, indicating it could be a promising agent for synthetic applications. (C) 2010 Elsevier Ltd. All rights reserved.