A new biobleaching sequence, with two enzymatic stages based on the application of laccase-mediator systems, was tested (L(1)EL(2)QPo) in order to increase the effectiveness of enzyme delignification on eucalypt kraft pulp Different synthetic - 1-hydroxybenzotriazole (HBT) and violuric acid (VA) - and natural syringaldehyde (SyAl) - mediators were used in the laccase stages and the biobleached pulp were compared in terms of chemical, optical and physico-mechanical properties. The pulp bleached with HBT or VA showed similar delignification (64.1% and 659% respectively) and optical properties (86 4% and 86 1% ISO brightness respectively) than an industrial TCF pulp (68 3% delignification and 848% ISO brightness) SyAl improved these properties in a lower extent (56 71% delignification and 8052% ISO brightness). Regarding physico-mechanical properties of pulp, the biobleaching sequence had no a negative effect, even some slight improvements were observed in very specific cases (C) 2010 Elsevier Ltd All rights reserved