The enzymatic reduction of Cr(VI) to Cr(III) by Cr(VI) resistant bacteria followed by chemical precipitation constitutes the ChromeBac (TM) system. Acinetobacter haemolyticus was immobilized onto carrier material inside a 0.2 m(3) bioreactor. Neutralized electroplating wastewater with Cr(VI) concentration of 17-81 mg L(-1) was fed into the bioreactor (0.11-0.33 m(3) h(-1)). Complete Cr(VI) reduction to Cr(III) was obtained immediately after the start of bioreactor operation. Together with the flocculation, coagulation and filtration, outflow concentration of less than 0.02 mg Cr(VI) L(-1) and 1 mg total Cr L(-1) were always obtained. Performance of the bioreactor was not affected by fluctuations in pH (6.2-8.4), Cr(VI) (17-81 mg L(-1)), nutrient (liquid pineapple waste, 1-20% v/v) and temperature (30-38 degrees C). Standby periods of up to 10 days can be tolerated without loss in activity. A robust yet effective biotechnology to remove chromium from wastewater is thus demonstrated. (C) 2010 Elsevier Ltd. All rights reserved.